| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha : |
15/08/2022 |
|
Actualizado : |
01/12/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
|
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
|
Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
|
Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
|
DOI : |
10.1002/mrd.23638 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
|
Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
|
Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 03782naa a2200409 a 4500
001 1063525
005 2022-12-01
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1002/mrd.23638$2DOI
100 1 $aPASSOS, J.R.S.
245 $aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil.
520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC.
653 $aFOLLICLE
653 $aOVARY
653 $aOVINE
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aPROTEINS
653 $aREPRODUCTION
700 1 $aGUERREIRO, D.D.
700 1 $aKAMILA S. OTÁVIO
700 1 $aDOS SANTOS-NETO, P.C.
700 1 $aSOUZA-NEVES, M.
700 1 $aCUADRO, F.
700 1 $aNUÑEZ?OLIVERA, R.
700 1 $aCRISPO, M.
700 1 $aVASCONCELOS, F.R.
700 1 $aBEZERRA, M.J.B.
700 1 $aSILVA, R.F.
700 1 $aLIMA, L.F.
700 1 $aFIGUEIREDO, J.R.
700 1 $aBUSTAMANTE-FILHO, I.C.
700 1 $aMENCHACA, A.
700 1 $aMOURA, A.A.
773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha actual : |
27/09/2022 |
|
Actualizado : |
27/09/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
BAEZA, S.; VÉLEZ-MARTIN, E.; DE ABELLEYRA, D.; BANCHERO, S.; GALLEGO, F.; SCHIRMBECK, J.; VERON, S.; VALLEJOS, M.; WEBER, E.; OYARZABAL, M.; BARBIERI, A.; PETEK, M.; GUERRA LARA, M.; SARRAILHÉ, S.S.; BALDI, G.; BAGNATO, C.; BRUZZONE, L.; RAMOS, S.; HASENACK, H. |
|
Afiliación : |
SANTIAGO BAEZA, Departamento de Sistemas Ambientales, Facultad de Agronomía, UdelaR, Montevideo, Montevideo, 12900, Uruguay; E. VÉLEZ-MARTIN, GeoKarten Consultoria em Tecnologia da Informação Ltda. Roca Sales, Rio Grande do Sul, 95735-000, Brazil; D. DE ABELLEYRA, Instituto de Clima y Agua, Instituto Nacional de Tecnología Agropecuaria (INTA) Hurlingham, Buenos Aires, 1686, Argentina; S. BANCHERO, Instituto de Clima y Agua, Instituto Nacional de Tecnología Agropecuaria (INTA) Hurlingham, Buenos Aires, 1686, Argentina; F. GALLEGO, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias, UdelaR, Montevideo, Montevideo, 11400, Uruguay; J. SCHIRMBECK, GeoKarten Consultoria em Tecnologia da Informação Ltda. Roca Sales, Rio Grande do Sul, 95735-000, Brazil; S. VERON, Instituto de Clima y Agua, Instituto Nacional de Tecnología Agropecuaria (INTA) Hurlingham, Bs. As., Argentina;Departamento de Métodos Cuantitativos y Sistemas de Información, LART, IFEVA, Facultad de Agronomía, UBA/CONICET, Bs. As., Argentina; MARÍA VALLEJOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. WEBER, Departamento Interdisciplinar e Programa de Pós-Graduação em Sensoriamento Remoto, Universidade Federal do Rio Grande do Sul, Campus Litoral Norte. Tramandaí, Rio Grande do Sul, 95590-000, Brazil; M. OYARZABAL, Departamento de Métodos Cuantitativos y Sistemas de Información, LART, IFEVA, Facultad de Agronomía, UBA/CONICET, Buenos Aires, Buenos Aires, 1417, Argentina; A. BARBIERI, Departamento de Geografía, Facultad de Ciencias, UdelaR, Montevideo, Montevideo, 11400, Uruguay; M. PETEK, Instituto de Clima y Agua, Instituto Nacional de Tecnología Agropecuaria (INTA) Hurlingham, Buenos Aires, 1686, Argentina; M. GUERRA LARA, Instituto de Matemática Aplicada San Luis, Universidad Nacional de San Luis y CONICET, San Luis, San Luis, 5700, Argentina; S. S. SARRAILHÉ, Instituto de Clima y Agua, Instituto Nacional de Tecnología Agropecuaria (INTA) Hurlingham, Buenos Aires, 1686, Argentina; G. BALDI, Instituto de Matemática Aplicada San Luis, Universidad Nacional de San Luis and CONICET, San Luis, San Luis, 5700, Argentina; C. BAGNATO, IRNAD, UNRN, CONICET, Río Negro, San Carlos de Bariloche, 8400, Argentina; L. BRUZZONE, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias, UdelaR, Montevideo, Montevideo, 11400, Uruguay; S. RAMOS, Departamento de Geografía, Facultad de Ciencias, UdelaR, Montevideo, Montevideo, 11400, Uruguay; H. HASENACK, Departamento de Ecologia, IB e Programa de Pós-Graduação em Agronegócios, CEPAN, Universidade Federal do Rio Grande do Sul. Porto Alegre, Rio Grande do Sul, 91501-970, Brazil. |
|
Título : |
Two decades of land cover mapping in the Río de la Plata grassland region: The MapBiomas Pampa initiative. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Remote Sensing Applications: Society and Environment, 2022, Volume 28, Article 100834. doi: https://doi.org/10.1016/j.rsase.2022.100834 |
|
ISSN : |
2352-9385 |
|
DOI : |
10.1016/j.rsase.2022.100834 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 26 April 2022; Received in revised form 29 August 2022; Accepted 3 September 2022; Available online 8 September 2022.
Corresponding author: Baeza, S.; Departamento de Sistemas Ambientales, Facultad de Agronomía, UdelaR, Montevideo, Montevideo, Uruguay; email:sbaeza@fagro.edu.uy -- This work has been also partially funded by ANII INNOVAGRO projects FSA_PI_2018_1_149022 and FSA_PI_2018_1_148811; CSIC I+D 2020_358, FMV_3_2020_1_162279, and FMV_1_2021_1_167032. |
|
Contenido : |
ABSTRACT.- The Río de la Plata Grasslands (RPG) region is the largest area of the temperate humid and sub-humid grasslands biome in South America and one of the largest in the world. The region is located on fertile soils, generally very suitable for agricultural development, so it is undergoing an intense land cover change process. Our knowledge of these changes remains incomplete. Most regional-scale studies have been conducted over specific periods, limited subsets of the RGP, coarse resolution and, in general, used land cover classes that are not readily compatible. In this work we described and analyzed the land cover changes in the entire RPG region for the first two decades of the 21st century, especially those related to grasslands loss. We generated annual land cover maps with 30-m resolution that discriminate between 8 categories: native woody formation, forest plantation, swampy areas and flooded grassland, grassland, farming, non-vegetated area, water and non-observed. The map series was evaluated for the years 2001 and 2018 using a completely independent dataset, selected by stratified randomized sampling. Overall accuracy was 73.5% and 77.8% for 2001 and 2018, respectively, with user and producer accuracies that varied between classes and years. In 20 years, RPG region lost, at least, 2.4 million ha of grassland (9% of the remaining grassland area in 2001). Most of these losses are concentrated in Brazil and Uruguay and are associated with new agricultural or forestry areas that increased by 5% and 100%, respectively. Our maps allow a comprehensive understanding of the transformation processes that RPG are undergoing and provide the context on which to explore a large set of hypotheses related to ecosystem structure and functioning. It will also contribute to improving decision-making at both the regional and national levels. © 2022 Elsevier B.V. MenosABSTRACT.- The Río de la Plata Grasslands (RPG) region is the largest area of the temperate humid and sub-humid grasslands biome in South America and one of the largest in the world. The region is located on fertile soils, generally very suitable for agricultural development, so it is undergoing an intense land cover change process. Our knowledge of these changes remains incomplete. Most regional-scale studies have been conducted over specific periods, limited subsets of the RGP, coarse resolution and, in general, used land cover classes that are not readily compatible. In this work we described and analyzed the land cover changes in the entire RPG region for the first two decades of the 21st century, especially those related to grasslands loss. We generated annual land cover maps with 30-m resolution that discriminate between 8 categories: native woody formation, forest plantation, swampy areas and flooded grassland, grassland, farming, non-vegetated area, water and non-observed. The map series was evaluated for the years 2001 and 2018 using a completely independent dataset, selected by stratified randomized sampling. Overall accuracy was 73.5% and 77.8% for 2001 and 2018, respectively, with user and producer accuracies that varied between classes and years. In 20 years, RPG region lost, at least, 2.4 million ha of grassland (9% of the remaining grassland area in 2001). Most of these losses are concentrated in Brazil and Uruguay and are associated with new agricultural or f... Presentar Todo |
|
Palabras claves : |
Classification; Grasslands; Land use change; Landsat; Time series. |
|
Asunto categoría : |
F01 Cultivo |
|
Marc : |
LEADER 03626naa a2200433 a 4500
001 1063583
005 2022-09-27
008 2022 bl uuuu u00u1 u #d
022 $a2352-9385
024 7 $a10.1016/j.rsase.2022.100834$2DOI
100 1 $aBAEZA, S.
245 $aTwo decades of land cover mapping in the Río de la Plata grassland region$bThe MapBiomas Pampa initiative.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 26 April 2022; Received in revised form 29 August 2022; Accepted 3 September 2022; Available online 8 September 2022. Corresponding author: Baeza, S.; Departamento de Sistemas Ambientales, Facultad de Agronomía, UdelaR, Montevideo, Montevideo, Uruguay; email:sbaeza@fagro.edu.uy -- This work has been also partially funded by ANII INNOVAGRO projects FSA_PI_2018_1_149022 and FSA_PI_2018_1_148811; CSIC I+D 2020_358, FMV_3_2020_1_162279, and FMV_1_2021_1_167032.
520 $aABSTRACT.- The Río de la Plata Grasslands (RPG) region is the largest area of the temperate humid and sub-humid grasslands biome in South America and one of the largest in the world. The region is located on fertile soils, generally very suitable for agricultural development, so it is undergoing an intense land cover change process. Our knowledge of these changes remains incomplete. Most regional-scale studies have been conducted over specific periods, limited subsets of the RGP, coarse resolution and, in general, used land cover classes that are not readily compatible. In this work we described and analyzed the land cover changes in the entire RPG region for the first two decades of the 21st century, especially those related to grasslands loss. We generated annual land cover maps with 30-m resolution that discriminate between 8 categories: native woody formation, forest plantation, swampy areas and flooded grassland, grassland, farming, non-vegetated area, water and non-observed. The map series was evaluated for the years 2001 and 2018 using a completely independent dataset, selected by stratified randomized sampling. Overall accuracy was 73.5% and 77.8% for 2001 and 2018, respectively, with user and producer accuracies that varied between classes and years. In 20 years, RPG region lost, at least, 2.4 million ha of grassland (9% of the remaining grassland area in 2001). Most of these losses are concentrated in Brazil and Uruguay and are associated with new agricultural or forestry areas that increased by 5% and 100%, respectively. Our maps allow a comprehensive understanding of the transformation processes that RPG are undergoing and provide the context on which to explore a large set of hypotheses related to ecosystem structure and functioning. It will also contribute to improving decision-making at both the regional and national levels. © 2022 Elsevier B.V.
653 $aClassification
653 $aGrasslands
653 $aLand use change
653 $aLandsat
653 $aTime series
700 1 $aVÉLEZ-MARTIN, E.
700 1 $aDE ABELLEYRA, D.
700 1 $aBANCHERO, S.
700 1 $aGALLEGO, F.
700 1 $aSCHIRMBECK, J.
700 1 $aVERON, S.
700 1 $aVALLEJOS, M.
700 1 $aWEBER, E.
700 1 $aOYARZABAL, M.
700 1 $aBARBIERI, A.
700 1 $aPETEK, M.
700 1 $aGUERRA LARA, M.
700 1 $aSARRAILHÉ, S.S.
700 1 $aBALDI, G.
700 1 $aBAGNATO, C.
700 1 $aBRUZZONE, L.
700 1 $aRAMOS, S.
700 1 $aHASENACK, H.
773 $tRemote Sensing Applications: Society and Environment, 2022, Volume 28, Article 100834. doi: https://doi.org/10.1016/j.rsase.2022.100834
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
